Personal profile

Junchao Dong received his B.S. from Fudan University, Ph.D. from the University of Texas Health Science Center at SAN Antonio, and postdoctoral research at Harvard Medical School. Supported by the National High-level Talent Introduction Youth Project, the Innovation and Entrepreneurship Team of Guangdong Pearl River Talent Plan and Sun Yat-sen University, she has been a professor of Sun Yat-sen School of Medicine of Sun Yat-sen University since 2017, focusing on the development, maturity and pathological changes of lymphocytes. It focuses on the multi-omics approach to study the mechanisms of epigenetic regulation and three-dimensional genome structure affecting lymphocyte development and hematological tumorigenesis, and explore new therapies for tumor immunotherapy. Relevant research results have been published in Nature, Molecular Cell, EMBO J., PNAS, Cell Reports, J. Leukocyte. Biol. And other academic journals.

Academic achievement

1. Wanyu Bai,  Guangchao Zhu, Jiejie Xu,  Pingyue Chen,  Feilong Meng, Hongman Xue, Chun Chen# and Junchao Dong#. 3’-flap endonuclease XPF-ERCC1 promotes alternative end joining and chromosomal translocation during B cell class switching. Cell Reports. In press.

2. X Sun, M Liu, J Bai, J Xu, C Zhu, J Dong#, C Chen#.  ATR kinase activity promotes antibody class switch recombination in B cells through cell cycle regulation without suppressing DSB resection and microhomology usage. J. Leukoc Biol. 2021 Apr 22

3. Dingpeng Yang, Ying Sun, Jingjing Chen, Ying Zhang, et al.,  REV7 is required for processing AID initiated DNA lesions in activated B cells. Nature Communications. 2020(1):1-11

4. Xiaojing Liu, Tingting Liu, Yafang Shang, Pengfei Dai, Wubing Zhang, et al., ERCC6L2 promotes DNA orientation-specific recombination in mammalian cells. Cell Research, 2020:1-13

5. Guoxing Zheng, Qingqing Zhu, Junchao Dong, Xin Lin, Chengming Zhu. Rapid generation and selection of Cas9-engineering TRP53 R172P mice that do not have off-target effects. BMC Biotechnology. 2019

6. Juan He, Qiong Yang, Qiang Xiao, Aihua Lei, Xing Li, Pan Zhou, Ting Liu, Lijuan Zhang, Kun Shi, Quan Yang, Junchao Dong, Jie Zhou. IRF-7 Is a Critical Regulator of Type 2 Innate Lymphoid Cells in Allergic Airway Inflammation. Cell reports 2019: 29 (9), 2718-2730 

7. Panchakshari RA, Zhang X, Kumar V, Du Z, Wei PC, Kao J, Dong J#, Alt FW#. DNA double-strand break response factors influence end-joining features of IgH class switch and general translocation junctions. Proc Natl Acad Sci U S A. 2018 Jan 23;115(4):762-767. (*co-first author)

8. Nguyen HV, Dong J, Panchakshari RA, Kumar V, Alt FW, Bories JC. Histone methyltransferase MMSET promotes AID-mediated DNA breaks at the donor switch region during class switch recombination. Proc Natl Acad Sci U S A. 2017 Dec 5;114(49)

9. Hu J, Meyers RM, Dong J, Panchakshari RA, Alt FW, Frock RL. Detecting DNA double-stranded breaks in mammalian genomes by linear amplification-mediated high-throughput genome-wide translocation sequencing. Nat Protoc. 2016 May;11(5):853-71

10.  Dong J#, Panchakshari RA#, Zhang T#, Zhang Y, Hu J, Volpi SA, Meyers RM, Ho YJ, Du Z, Robbiani DF, Meng F, Gostissa M, Nussenzweig MC, Manis JP, Alt FW. Orientation-specific joining of AID-initiated DNA breaks promotes antibody class switching. Nature. 2015 Sep 3;525(7567):134-9 (#co-first author)

11. Gostissa M, Schwer B, Chang A, Dong J, Meyers RM, Marecki GT, Choi VW, Chiarle R, Zarrin AA, Alt FW. gH class switching exploits a general property of two DNA breaks to be joined in cis over long chromosomal distances. Proc Natl Acad Sci U S A. 2014 Feb 18;111(7):2644-9

12. Li F#, Dong J#, Eichmiller R, Holland C, Minca E, Prakash R, Sung P, Shim E, Surtees JA, Eun Lee S. Role of Saw1 in Rad1/Rad10 complex assembly at recombination intermediates in budding yeast. EMBO J. 2013 Feb 6;32(3):461-72 (#co-first author)

13. Toh GW#, Sugawara N#, Dong J#, Toth R, Lee SE, Haber JE, Rouse J.Mec1/Tel1-dependent phosphorylation of Slx4 stimulates Rad1/Rad10 dependent cleavage of non-homologous DNA tails. DNA Repair (Amst). 2010 Jun 4;9(6):718-2 (#co-first author)

14. Li F, Dong J#, Pan X, Oum JH, Boeke JD, Lee SE. Microarray- based genetic screen defines SAW1, a gene required for Rad1/Rad10-dependent processing of recombination intermediates.Mol Cell. 2008 May 9;30(3):325-35 (#co-first author)

Scientific research project

Fund Project:
1. Guangdong Province introduced innovation and entrepreneurship team, team leader, iPS cell-derived tissue construction and research and development of new technologies for the treatment of major diseases, project number: 2016ZT06S029, year: 2017-202
2. Key Project of Natural Science Foundation of Guangdong Province, Host, Molecular mechanism of breast cancer occurrence, development, drug resistance and metastasis based on iPS cell breast cancer model, Project number: 2021B1515120063, year: 2021-2024
3. Sponsored by the National Natural Science Foundation of China, Cell fate-determining transcription factors regulate cell fate by inducing TAD recombination of chromatin three-dimensional structure. Project approval number: 32170798, Year: 2022-2025
4. National Natural Science Foundation of China (NSFC), Director, Function and mechanism of nuclear spatial epigenetic domain regulating neural differentiation. Project Approval number: 31970811, Year: 2020-2023
5. National Natural Science Foundation of China (NSFC), Host, Function and mechanism of DNA-binding protein Arid3b in the establishment of pluripotency and Directed differentiation of stem cells, project approval number: 31771639, year: 2018-2021

Award honor

2016 Selected into the second batch of Sun Yat-sen University
2016 Selected as the national high-level talent Introduction youth project
2017 Selected in Guangdong Province \"Pearl River Talent Plan\" to introduce innovation and entrepreneurship team
2017 Backbone of key R&D projects of the Ministry of Science and Technology

Academic part-time job

Invited reviewers for the following academic journals:
Cancer Letters (impact factor 5.62)
Molecular and Cellular Biology (impact factor 4.78)
International Journal of Biological Sciences (3.98)
PLOS One(Impact factor 3.23)
Mutation Research (Impact factor 3.68)