个人简介

2025-至今：教授，中山医学院，中山大学

2015-2024：副教授，中山医学院，中山大学

2011.6-2014.12:  博士后,微生物和分子遗传系,医学院,美国加州大学欧文分校

2009.6- 2011.5:  博士后,化学与生物化学系,美国加州大学圣地亚哥分校

2003.9-2009.5:  博士研究生,上海生物化学与细胞生物学研究所,中国科学院

1999.9-2003.7:  本科生,生物科学系,南开大学,中国天津

研究方向 

真核复杂类型细胞如干细胞/免疫细胞等基因转录后调控的机制研究正越来越受到研究者的关注。相比转录水平（组蛋白修饰，DNA修饰，转录因子等），转录后调控（RNA加工，稳定性调控等）对于细胞来说，它消耗能量更少，更便于细胞精细调控，满足复杂细胞应对发育分化/抗炎应急等外来信号的需求。本研究组近期围绕mRNA APA(alternative polyadenylation)的机制和生物学功能意义展开以下科学问题的探索。

1. 非编码RNA调控mRNA3'末端加工和APA的机制

2. mRNA3'末端加工因子或APA调控因子是否参与干细胞命运决定过程

3. mRNA3'末端加工因子或APA调控因子是否参与某些病理过程

学术成果

主要学术研究成果与贡献

（1）首次发现U4/U6/U2 snRNP具有类似U1抑制pre-mRNA PCPA (premature cleavage and polyadenylation)和PTT(premature transcription termination)的功能，从而在共转录加工的分子机制领域提出普适性模型：pre-mRNA splicing在转录组规模可防止PCPA/PTT，从而正调控transcription。相关结果以最后通讯作者发表在国际权威期刊《PNAS》2024和《Cell Reports》2025上。

（2）2023年在生化领域权威期刊《JBC》上以最后通讯作者发表U1 snRNP telescripting的分子机制论文，为U1抑制pre-mRNA PCPA与PTT带来最新的见解。

（3）2017年在国际知名刊物《NAR》以最后通讯作者发表snoRNA概念与功能突破性进展研究，Article被评为NAR Breakthrough Article.首次揭示snoRNA可能在真核生物mRNA3'末端加工中发挥功能。

学术论著与教材

  1. Qiumin Feng, Zejin Lin, Danhui Zhao, Mengzhao Li, Sheng Yang, Andy Peng Xiang, Congting Ye*, Chengguo Yao* (2025) Functional inhibition of core spliceosomal machinery activates intronic premature cleavage and polyadenylation of pre-mRNAs. Cell Reports. Mar 25. 
  2. Qiumin Feng, Danhui Zhao, Zejin Lin, Mengzhao Li, Andy Peng Xiang, Congting Ye*, Chengguo Yao* (2024) U4 snRNP inhibits premature cleavage and polyadenylation of pre-mRNAs. PNAS. June 25.
  3. Qiumin Feng, Zejin Lin, Yanhui Deng, Yi Ran, Rui Yu, Andy Peng Xiang, Congting Ye*, Chengguo Yao* (2023) The U1 antisense morpholino oligonucleotide (AMO) disrupts U1 snRNP structure to promote intronic PCPA modification of pre-mRNAs. JBC. Available online 22 May 2023, 104854.
  4. Ran, Yi; Huang, Shanshan; Shi,Junjie; Feng, Qiumin; Deng,Yanhui; Xiang AP; Yao, Chengguo* (2022) CFIm25 regulates human stem cell function independently of its role in mRNA alternative polyadenylation. RNA Biology 19(1):686-702.
  5. Yixian Cun, Sanqi An, Haiqing Zheng, Jing Lan, Wenfang Chen, Wanjun Luo, Chengguo Yao, Xincheng Li, Xiang Huang, Xiang Sun, Zehong Wu, Yameng Hu, Ziwen Li, Shuxia Zhang, Geyan Wu, Meisongzhu Yang, Miaoling Tang, Ruyuan Yu, Xinyi Liao, Guicheng Gao, Wei Zhao, Jinkai Wang, Jun Li (2021）Specific Regulation of m 6 A by SRSF7 Promotes the Progression of Glioblastoma. Genomics Proteomics Bioinformatics . S1672-0229(21)00252-7.
  6. Ran, Yi; Deng, Yanhui; Chengguo Yao*(2021) U1 snRNP telescripting: molecular mechanisms and beyond. RNA Biology 18(11):1512-1523.
  7. Deng, Yanhui, Shi, Junjie; Ran, Yi; Xiang AP*; Yao, Chengguo* (2020) A potential mechanism underlying U1 snRNP inhibition of the cleavage step of mRNA 3' processing. BBRC 2020, Sep 2. 196-202.
  8. Shi, Junjie; Deng, Yanhui; Huang, Shanshan; Huang Chunliu; Wang jinkai; Xiang AP*; Yao, Chengguo* (2019) Suboptimal RNA-RNA interaction limits U1 snRNP inhibition of canonical mRNA 3' processing. RNA Biology 2019 Oct;16(10):1448-1460.
  9. Chang, Jae-Woong; Zhang, Wei;Yeh, Hsin Sung; Park, Meeyeon; Yao, Chengguo; Shi, Yongsheng; Kuang, Rui; Yong, Jeongsik.(2018)An integrative model for alternative polyadenylation, IntMAP, delineates mTOR-modulated endoplasmic reticulum stress response.Nucleic Acids Research 2018 doi: 10.1093/nar/gky340
  10. Shi,Junjie;Huang, Chunliu;Huang,Shanshan; Yao Chengguo* (2018) snoRNAs associated with mRNA 3' processing complex: new wine in old bottle. RNA Biology doi: 10.1080/15476286.2017.1416278 
  11. Huang, Chunliu; Shi, Junjie; Guo, Yibin; Huang, Weijun; Huang, Shanshan; Ming, Siqi; Wu, Xingui; Zhang, Rui; Ding, Junjun; Zhao, Wei; Jia, Jie; Huang, Xi; Xiang, Peng; Shi, Yongsheng*; Yao, Chengguo* (2017) A snoRNA modulates mRNA 3’ end processing and regulates the expression of a subset of mRNAs. Nucleic Acids Research 2017(NAR Breakthrough Article) 
  12. Maliheh Movassat, Tara L. Crabb, Anke Busch, Chengguo Yao, Derrick J. Reynolds, Yongsheng Shi & Klemens J. Hertel (2016) Coupling between alternative polyadenylation and alternative splicing is limited to terminal introns. RNA Biology. DOI:10.1080/15476286.2016.1191727
  13. Serena L. Chan, Ina Huppertz, Chenguo Yao, James J. Moresco, John R. Yates III, Jernej Ule, Yongsheng Shi (2014) CPSF30 and Wdr33 directly bind to AAUAAA in mammalian mRNA 3’ processing. Genes & Dev. 28(21):2370-80.
  14. Brad Lackford *, Chengguo Yao*, Georgette M. Charles *, Lingjie Weng, Xiaofeng Zheng, Eun-A Choi, Xiaohui Xie, Ji Wan, Yi Xing, Johannes M, Freudenberg, Raja Jothi, Guang Hu,Yongsheng Shi (*contributed equally). Fip1 regulates mRNA alternative polyadenylation to promote stem cell selfrenewal. EMBO J. 2014 16;33(8):878-89.
  15. Chengguo Yao, Lingjie Weng, Yongsheng Shi. Global protein-RNA interaction mapping at single nucleotide resolution by iCLIP-seq. Methods Mol Biol. 2014, 1126: 399-410.
  16. Chengguo Yao, Yongsheng Shi. Global RNA polyadenylation mapping by PAS-seq. Methods Mol Biol. 2014, 1125:179-85.
  17. Li Wang, Yi-liang Miao, Xiaofeng Zheng, Brad Lackford, Bingying Zhou, Leng Han, Chengguo Yao, James Ward, Adam Burkholder, Inna Lipchina, David C. Fargo, Konrad Hochedlinger, Yongsheng Shi, Carmen J. Williams, and Guang Hu. The THO Complex Regulates Pluripotency Gene mRNA Export and Controls Embryonic Stem Cell Self-Renewal and Somatic Cell Reprogramming. Cell Stem Cell. 2013 13(6):676-90.
  18. Chengguo Yao, Eun-A Choi, Lingjie Weng, Xiaohui Xie, Ji Wan, Yi Xing, James J. Moresco, Patricia G. Tu, John R. Yates, Yongsheng Shi. Overlapping and distinct functions of CstF64 and CstF64t in mammalian mRNA 3’ processing. RNA. 2013 19(12):1781-90.
  19. Chengguo Yao, Jacob Biesinger, Ji Wan, Lingjie Weng, Yi Xing, Xiaohui Xie, Yongsheng Shi. Transcriptome-wide analyses of CstF64–RNA interactions in global regulation of mRNA alternative polyadenylation. Proc Natl Acad Sci U S A. 2012, 109 (46): 18773-8.
  20. Chengguo Yao, Janina E. Moretti, Peter E. Struss, Junaid A. Spall, Ulrich F. Muller. Arginine Cofactors on the Polymerase Ribozyme. PLoS One. 2011; 6(9): e25030.
  21. Chengguo Yao, Ulrich F Muller. Polymerase Ribozyme efficiency increased by G/T-rich DNA oligonucleotides. RNA. 2011, 17(7):1274-81.